Search results for "16s rDNA sequencing"

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A polyphasic approach to the identification of ochratoxin A-producing black Aspergillus isolates from vineyards in Sicily.

2008

Aspergillus strains belonging to section Nigri isolated during a two year survey in eight Sicilian vineyards located on the slopes of Mount Etna (Sicily, Italy) were analysed analyzed in order to characterize species responsible for ochratoxin A (OTA) contamination of grapes. The polyphasic approach permitted analysis of biodiversity of Aspergillus isolates in relation to their morphology, ochratoxigenicity and genetic variability. We assessed OTA production by A. carbonarius, A. niger, A. tubingensis and A. japonicus using an enzyme-linked immunosorbent assay. A. carbonarius isolates were the strongest OTA producers. A subset of 66 representative strains was selected for further DNA-based …

Ochratoxin AVeterinary medicineEnzyme-Linked Immunosorbent AssayFood ContaminationfAFLPMicrobiologylaw.inventionMicrobiologychemistry.chemical_compoundVineyard OTA A. carbonariusPCR identificationSpecies SpecificitylawDNA Ribosomal SpacerCluster AnalysisVitisGenetic variabilityDNA FungalOchratoxinAspergillus; Ochratoxin A; fAFLP; PCR identification; 16s rDNA sequencingPolymerase chain reactionPhylogenyAspergillusbiology16s rDNA sequencingAspergillus nigerOchratoxin AGeneral MedicineFungi imperfectiSequence Analysis DNAbiology.organism_classificationOchratoxinsRNA Ribosomal 5.8SAspergilluschemistryItalyAspergillus nigerRestriction fragment length polymorphismPolymorphism Restriction Fragment LengthFood ScienceInternational journal of food microbiology
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